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Description
Rat AQP5 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an aquaporin 5 (AQP5) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of aquaporin 5 (AQP5) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Aquaporin 5 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Aquaporin-5 is a protein encoded by the AQP5 gene. AQP5 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin-5 plays a role in the production of saliva, tears, and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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★★★★★ 5
Sharp Fit & Great Quality
Size: Medium, Color: Red
Great suit — fits well and looks even better in person.
The material feels high quality, the cut is clean, and it gives a sharp, professional look. Perfect for business settings, meetings, or any occasion where you want to stand out.
Definitely a solid choice if you’re looking for style and confidence at a great value.
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Reviewed in the United States on April 1, 2026
★★★★★ 5
WULFUL Men's 3 Piece Slim Fit Suit Set - Elevate Your Style with Timeless Sophistication
Size: XX-Large, Color: Navy
The WULFUL Men's 3 Piece Slim Fit Suit Set has exceeded expectations, delivering a perfect blend of modern elegance, impeccable tailoring, and versatile style. This review reflects my positive experience with this distinguished suit ensemble.
Fit and Tailoring:
The slim fit design of the suit strikes a harmonious balance between a tailored silhouette and comfortable wear. The jacket, pants, and vest are expertly tailored, accentuating the body's natural lines without feeling restrictive. The attention to detail in the stitching and construction speaks volumes about the quality craftsmanship.
Fabric and Comfort:
Crafted from high-quality materials, the fabric of the suit feels luxurious against the skin. The breathable and lightweight nature ensures comfort throughout the day, making it suitable for a range of occasions. Whether worn for business meetings, special events, or formal gatherings, the suit maintains a comfortable and polished feel.
Timeless Style:
The classic design of the suit exudes timeless style, making it a versatile addition to any wardrobe. The subtle details, such as the notched lapel, welted pockets, and single-breasted closure, contribute to the overall sophistication. The suit effortlessly transitions from formal occasions to semi-formal events, showcasing its adaptability.
Versatility:
The versatility of this 3-piece suit set is a standout feature. The jacket, vest, and pants can be worn together for a complete formal look or styled separately for a more casual ensemble. This adaptability adds value to the suit set, allowing for a range of outfit options tailored to different occasions.
Attention to Detail:
WULFUL has demonstrated a keen eye for detail in the design and construction of this suit set. From the carefully aligned patterns to the precise tailoring of pockets and seams, the meticulous attention to detail elevates the overall aesthetic. The suit exudes a polished and refined appearance, suitable for any discerning gentleman.
Affordability and Value:
Considering the exceptional quality, craftsmanship, and timeless style, the affordability of the WULFUL Men's 3 Piece Slim Fit Suit Set is noteworthy. The set offers remarkable value for its price, making it an accessible option for individuals seeking a sophisticated wardrobe staple without compromising on quality.
Overall Impression:
In conclusion, the WULFUL Men's 3 Piece Slim Fit Suit Set has earned its place as a wardrobe essential. With its impeccable fit, luxurious fabric, timeless style, versatility, attention to detail, and affordable pricing, this suit set stands out as a stellar choice for those who appreciate both classic elegance and modern refinement. Elevate your style effortlessly with WULFUL, where sophistication meets affordability in a tailored masterpiece. Highly recommended for anyone seeking a distinguished and versatile suit ensemble!
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Reviewed in the United States on March 7, 2024
★★★★★ 5
Affordable and classy
Size: Medium, Color: Light Brown, Size: Medium, Color: Light Brown
I have five stars even if the best was a little short. I have a tall boy and the rest is totally worth the money. Fit was as marked aside from vest which is higher on the chest than necessary. It was comfortable for him and durable. Gonna keep for any need in the future. He wore it to prom and it was awesome.
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Reviewed in the United States on May 19, 2026
★★★★★ 4
3 piece suit at a great price
Size: Small, Color: Black
My son needed a suit, and these worked out great. It looks nice. Don't just assume the size, go by their size chart. I was going to order him a medium, by the chart recommended a small, and it fit perfect. The material doesn't look like an expensive suit but it worked out great for him.
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Fits so good and looks really niceeee
Size: Small, Color: White
Really nice suit. Looks super nice and high quality. Would purchase again.
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Reviewed in the United States on May 18, 2026